Bstfa silylation reagent
WebPhysical and Chemical Properties: Use: Silylation Reagent for GC Appearance: Clear, colorless liquid Purity: 98% Boiling Point: 147°C (296°F) Molecular Formula: C₈H₁₈F₃NOSi₂ + (CH₃)₂SiCl Molecular Weight: 257.40 g/mol (BSTFA) + 108.66 g/mol (TMCS) Specific Gravity (H 2 O = 1.0): 0.969 Suggested Storage Conditions: WebDec 5, 2024 · Silylation reagents can be used pure or in mixtures of two or even three reagents. The reagent mixtures may provide a more efficient silylation for specific compounds. For example, silylation of 3,4-dimethoxyphenylethylamine with BSA leads to the substitution of only one active hydrogen in the NH 2 group, while the silylation with …
Bstfa silylation reagent
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WebN,O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA) containing 1%trimethylchlorosilane was employed to derivatize amino groups by heating the reagent containing a sample of CSC at 80 °C for 30 min followed by analysis employing gas chromatography-mass spectroscopy (GC-MS) in the selected-ion-monitoring (SIM) mode. WebBSTFA can be used at full strength or diluted with a suitable solvent such as pyridine. In most applications it is advisable to use an excess of the silylating reagent and > 2:1 molar ratio of BSTFA to active hydrogen is optimal. Best results are obtained when the products of the silylation reaction are soluble in the final reaction mixture.
WebThis silylation reagent reacts with a wide range of polar compounds and is particularly well suited for the GC analyses of some lower boiling TMS-amino acids and TMS Krebs cycle acids. BSTFA is a powerful thrimethylsilyl donor, with donor strength that is comparable to its unfluorinated analog BSA [N,O-Bis(trimethylsilyl)acetamide]. WebJul 23, 2024 · All solvents were purchased from Sigma (Steinheim, Germany). Only Folin–Ciocalteu reagent and silylation reagents were purchased from Merck (Darmstadt, Germany), Water was supplied by a Milli-Q water purifier system from Millipore (Bedford, MA, USA). ... BSTFA was also used in similar cases such as wines and white juices .
WebBSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) is the pre- ferred reagent for trimethylsilylation of alcohols, alkaloids, amines and biogenic amines, carboxylic acids, … WebPhysical and Chemical Properties: Use: Silylation Reagent for GC Appearance: Clear, colorless liquid Purity: 98% Boiling Point: 147°C (296°F) Molecular Formula: C₈H₁₈F₃NOSi₂ + (CH₃)₂SiCl Molecular Weight: 257.40 g/mol (BSTFA) + 108.66 g/mol (TMCS) Specific Gravity (H 2 O = 1.0): 1.028 Suggested Storage Conditions:
BSTFA is a powerful thrimethylsilyl donor, with donor strength that is comparable to its unfluorinated analog BSA [N,O-Bis(trimethylsilyl)acetamide]. BSTFA reacts to replace labile hydrogens on a wide range of polar compounds with a -Si(CH 3) 3 group. This physical characteristic is particularly useful in the gas chromatography of some lower ...
WebN,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) is a chemical compound that is used to derivatise labile groups such as hydroxyl on other chemicals, with the more stable … tide and moonWebBSTFA is very versatile, reacting with a range of polar organic compounds and replacing active hydrogens with a −Si (CH 3) 3 (trimethylsilyl) group. Reacts rapidly … the mac martWebUse Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent for analytes that are difficult to silylate. Stronger than BSTFA alone, this catalyzed formulation is ideal for derivatizing compounds such as fatty acid amides and slightly hindered hydroxyls for GC analysis. Excellent for… the mac man moore okWebSilylation Reagents Chemical compounds designed to aid in mass spectrometric compound identification via silylation. Filter By Chromatography Supplies BSTFA +1% … tide and offeringWebIn most cases, the silylation reagent is used in large excess. Therefore, very small amounts of moisture in the sample can be tolerated, as the water will react with the reagent and be removed chemically from the system. Hydrolysis of TMS derivatives and reagents produces hexamethyldisiloxane [(CH3)3SiOSi(CH3)3]. A small amount of the siloxane ... the mac metropolitan arts centreWebThe silylation by The presence of transesterification products in samples subjected both BSTFA and MTBSTFA resulted in extraordinary precision of the to hydrolysis using KOH solution in methanol was not a trouble results, when compared with methylation procedures. tide and moon phase chartWebDec 15, 2011 · Thus, despite BSTFA being associated with trimethylchlorosilane, also a silylation reagent, the same concentration of CP and PS the chromatographic m/z peak ions of BSTFA derivates are smaller than those obtained with MSTFA (Fig. 3). This finding is due to the MSTFA association with trimethyliodosilane (a more potent and selective ... tide and purl